Detection of Wolbachia (Rickettsiales: Anaplasmataceae) and Candidatus Liberibacter asiaticus (Rhizobiales: Rhizobiaceae) Associated With Diaphorina citri (Hemiptera: Liviidae) Collected From Citrus reticulata (Sapindales: Rutaceae) and Alternate Host, Cordia myxa (Boraginales: Boraginaceae)

Güz N., Arshad M., Cagatay N. S., Dageri A., Ullah M. I.

JOURNAL OF ECONOMIC ENTOMOLOGY, cilt.113, sa.3, ss.1486-1492, 2020 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 113 Sayı: 3
  • Basım Tarihi: 2020
  • Doi Numarası: 10.1093/jee/toaa043
  • Dergi Adı: JOURNAL OF ECONOMIC ENTOMOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Agricultural & Environmental Science Database, Animal Behavior Abstracts, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, CAB Abstracts, EMBASE, Environment Index, MEDLINE, Veterinary Science Database, DIALNET
  • Sayfa Sayıları: ss.1486-1492
  • Anahtar Kelimeler: Diaphorina citri, Citrus reticulata, Cordia myxa, 'Candidatus Liberibacter asiaticus', endosymbionts, INFECTION DENSITY, GREENING DISEASE, ENDOSYMBIONTS, PSYLLIDAE, TRANSMISSION, POPULATIONS, DIVERSITY, SYMBIONT, PROLIFERATION, SPIROPLASMA
  • Ankara Üniversitesi Adresli: Evet

Özet

The Asian citrus psyllid, Diaphorina citri Kuwayama (Hemiptera: Liviidae), is an important insect pest of the citrus crop worldwide. It vectors the pathogen 'Candidatus Liberibacter asiaticus' (CLas) that causes a serious disease known as citrus greening. Here, we tested the infection frequency of Wolbachia and CLas from 100 D. citri individuals collected from two host plants belonging to families Rutaceae (Citrus reticulata Blanco) and Boraginaceae (Cordia myxa L.) using molecular methods. The following trend of endosymbionts infection in adult D. citri was found; 85.4% (35/41) by Wolbachia, and 19.5% (8/41) by CLas collected from C. reticulata plants and 65.4% (17/26) by Wolbachia, and 15.4% (4/26) by CLas in case of C. myxa plant. However, 61.5% (8/13) nymphs collected from C. reticulata and 20.0% (4/20) collected from C. myxa plants were infected by Wolbachia, while no nymph was infected by CLas collected from either host plants. Findings from this work represent the first report of CLas presence in D. citri feeding on C. myxa plants. By studying the presence of CLas with other endosymbiotic bacteria, future basic and applied research to develop control strategies can be prioritized.