1st European Symposium on Animal Reproduction, 21st – 23rd September 2023, Nantes, France, Nantes, Fransa, 21 - 24 Eylül 2023, cilt.58, ss.273-274
Angora bucks and 52 primiparous does were used. The three step selection was performed according to mohair quality, integrated an- drological examination system and fertility. A total of 55 ejaculates from 5 bucks were collected with an artificial vagina. The samples were diluted with Tris egg yolk diluent (without removal of seminal plasma) to a final concentration of 200 × 106 sperm per straw and frozen with liquid nitrogen. Fresh sperm motility and mass activ- ity were evaluated with phase-contrast microscope and post-thaw spermatological analysis with CASA. The sperm viability was done with duovital and morphology with sperm blue stain. For the esti- mation of fertility, does were synchronized in breeding season with P4 sponge for 11-day protocol. Estrus signs were detected, and transcervical inseminations (TCI) were carried out 43 h and laparo- scopic (LAI) 56 h after the sponge removal. For each of the males, 10 inseminations were performed (5 LAI & 5 TCI) with frozen semen. Each variable was investigated for their effect on pregnancy using Student's t- and Chi Square tests depending on the nature of the variable. There was a statistical difference between the TCI (33.3%) and the LAI (66.7%) method in terms of pregnancy rate (p < 0.05). The statistical differences were detected between the pregnant and non-pregnant groups in terms of kinetic parameters such as linearity (38 vs. 40.62), wobble (61.29 vs. 64.41), vitality (53.34 vs. 59.51) and acrosome integrity (64.21 vs. 71.87 values. This selection system can be used to eliminate the undesirable characteristics and ensures the fertility outcome for semen banking.