Proteomics analysis of the Flp regulon in Lactococcus lactis subsp cremoris

Akyol İ.

ELECTROPHORESIS, cilt.34, sa.15, ss.2218-2228, 2013 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 34 Sayı: 15
  • Basım Tarihi: 2013
  • Doi Numarası: 10.1002/elps.201300002
  • Dergi Adı: ELECTROPHORESIS
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.2218-2228
  • Anahtar Kelimeler: flpA, flpB, Lactococcus lactis, Proteomics, COMPLETE GENOME SEQUENCE, COLD SHOCK PROTEINS, PSEUDOMONAS-AERUGINOSA, ACID BACTERIA, ELECTRON-TRANSFER, GENE, ARGININE, OPERON, IDENTIFICATION, MG1363
  • Ankara Üniversitesi Adresli: Hayır

Özet

Lactococcus lactis subsp. cremorisMG1363 genome sequence was completed and encodes two flp genes flpA and flpB. Research carried out has suggested that the flpB proteins are transcriptional regulators that respond to the environmental oxygen level. A variety of flp deletion mutant strains with single and double mutation were created. Wild-type (MG1363) and its flp(-) derivatives were compared by 2DE to identify changes in protein intensity under different aerobic/anaerobic growth conditions. In total, 416 +/- 20 and 444 +/- 32 protein spots were quantified from anaerobic and aerobic cells, respectively, on pH 4-7 gels. Forty-five protein spots that changed were excised from 2DE gel, digested with trypsin and identified from their MALDI- eptide Mass Fingerprint. A variety of proteins were affected by the flp mutations and oxygen level. Some proteins were controlled by FlpA and FlpB independently and some required both FlpA and B for regulation. Flp proteins can be grouped by biochemical function. ABC transporters, arginine metabolism, and pyrimidine biosynthesis pathway.