DNA barcoding of sunn pest adult parasitoids using cytochrome c oxidase subunit I (COI)

Duman M., Güz N., Sertkaya E.

BIOCHEMICAL SYSTEMATICS AND ECOLOGY, cilt.59, ss.70-77, 2015 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 59
  • Basım Tarihi: 2015
  • Doi Numarası: 10.1016/j.bse.2015.01.003
  • Dergi Adı: BIOCHEMICAL SYSTEMATICS AND ECOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.70-77
  • Anahtar Kelimeler: Adult parasitoids, Cytochrome c oxidase subunit I, DNA barcoding, Sunn pest, INTEGRICEPS PUT. HEMIPTERA, POPULATION, PHYLOGENIES, RESISTANCE, SEQUENCE, GENE
  • Ankara Üniversitesi Adresli: Evet

Özet

In this study, DNA barcoding was used in the identification of potential biological control agents of sunn pest adult parasitoid species, including Eliozeta helluo (F.), Phasia subcoleoptrata (L.), Ectophasia crassipennis (F.) and Elomyia lateralis (Meig). DNA analyses were assessed by sequencing cytochrome c oxidase subunit I (COI) gene. The obtained sequences were analyzed in terms of nucleotide composition, nucleotide pair frequency and haplotype diversity. Genetic divergence among haplotypes was estimated by constructing genetic distance matrix using DNA sequence variations, by Kimura 2-parameter model. Variable sites and average variations of the sequenced 603 base pair long DNA fragment were calculated. All COI barcodes were matched with reference sequences of expected species according to morphological identification. Neighbor-joining tree was drawn based on DNA barcodes and all the specimens clustered in agreement with their taxonomic classification at species level. The evolutionary history inferred using the UPGMA method indicated two distinct mitochondrial haplotype lineages. The genetic variation between sunn pest adult parasitoids will be useful in sunn pest management, regulatory and environmental applications. (C) 2015 Elsevier Ltd. All rights reserved.