Molecular characterisation of hydrogen sulfide negativeSalmonella entericaserovar Havana

MÜŞTAK İ. B., MÜŞTAK H. K., SARIÇAM İNCE S.

ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY, cilt.113, sa.9, ss.1241-1246, 2020 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 113 Sayı: 9
  • Basım Tarihi: 2020
  • Doi Numarası: 10.1007/s10482-020-01432-3
  • Dergi Adı: ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Agricultural & Environmental Science Database, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, Biotechnology Research Abstracts, CAB Abstracts, EMBASE, Environment Index, Food Science & Technology Abstracts, MEDLINE, Veterinary Science Database
  • Sayfa Sayıları: ss.1241-1246
  • Anahtar Kelimeler: Hydrogen sulfide, Phs operon, Salmonella, FIELD GEL-ELECTROPHORESIS, ANTIMICROBIAL RESISTANCE, SALMONELLA-TYPHIMURIUM, SEROVAR TYPHIMURIUM, PREVALENCE, INFECTIONS, REDUCTION, PATTERNS
  • Ankara Üniversitesi Adresli: Evet

Özet

Hydrogen sulfide (H2S) detection is a screening method for distinguishing and identifyingSalmonellastrains from other bacteria in the intestine. Incidences of H2S-negativeSalmonellahave recently been reported in different countries. Although a high resistance rate against antimicrobial agents has been reported for H2S-positiveSalmonellain many regions of the world, there is increasing evidence that high resistance to antibiotics has also increased in many H2S-negativeSalmonellaisolates. In this study, molecular characterisation of three H2S-negativeSalmonellaHavana, isolated from cloacal swab samples of broiler chickens, was performed. ThephsA, phsBandphsCgenes of thephsoperon, which is responsible for hydrogen sulfide production, were amplified. Sequence analysis was then performed to identify mutations in the gene cluster. The antimicrobial resistance profiles of the isolates were determined by disc diffusion. Molecular characterisation was performed by multilocus sequence typing (MLST) and pulsed field gel electrophoresis (PFGE). The sequence analysis showed identified five point mutations in thephsAgene and one point mutation in thephsCgene in all isolates. The antibiotic resistance profile showed that the strains were resistant to cefoxitin and ceftazidime. MLST analysis showed that all strains belonged to sequence type (ST) 1621. This study is the first to report the H2S-negativeS.Havana serotype.