Effects of different serum types in in vitro culture of in vitro fertilized rabbit oocytes

Tekin N., Sariozkan S., UYSAL O., AKÇAY E.

ANKARA UNIVERSITESI VETERINER FAKULTESI DERGISI, cilt.54, sa.2, ss.87-90, 2007 (SCI-Expanded) identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 54 Sayı: 2
  • Basım Tarihi: 2007
  • Dergi Adı: ANKARA UNIVERSITESI VETERINER FAKULTESI DERGISI
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), TR DİZİN (ULAKBİM)
  • Sayfa Sayıları: ss.87-90
  • Anahtar Kelimeler: in vitro culture, in vitro fertilization, oocyte, rabbit, serum, EMBRYO DEVELOPMENT, INVITRO FERTILIZATION, BOVINE EMBRYOS, MEDIA, OVA, IMPLANTATION, BLASTOCYSTS, PROTEIN, MORULAE, STAGE
  • Ankara Üniversitesi Adresli: Evet

Özet

In this study 33 New Zealand White does and 2 bucks were used. After induction Of Ovulation by human chorionic gonadotrophin (hCG) injection, oocytes were collected by flushing of oviduct. Oocytes and spermatozoa were incubated for 5 hours in Modifie Defined Medium (mDM) for fertilization and cultured in Ham's F-10 medium supplemented with 20 % Fetal Calf Serum (FCS) and 20 % Rabbit Serum (RS) for 18-21 hours, to evaluate the effect of serum in fertilization. Fertilization was assessed by detection of oocytes cleavage rates. The fertilization rates were recorded as 70.4 %, 73.8 %, 60.6 % in oocytes incubated in culture mediums with FCS or RS or without any serum respectively (p>0.05). Data were evaluated via Chi-square test. These data suggest that the differences between group averages were not statistically significant, although the fertilization rate in Ham's F-10 with rabbit serum was higher.