Effects of different serum types in in vitro culture of in vitro fertilized rabbit oocytes
ANKARA UNIVERSITESI VETERINER FAKULTESI DERGISI, cilt.54, sa.2, ss.87-90, 2007 (SCI-Expanded, TRDizin)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 54 Sayı: 2
- Basım Tarihi: 2007
- Dergi Adı: ANKARA UNIVERSITESI VETERINER FAKULTESI DERGISI
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), TR DİZİN (ULAKBİM)
- Sayfa Sayıları: ss.87-90
- Anahtar Kelimeler: in vitro culture, in vitro fertilization, oocyte, rabbit, serum, EMBRYO DEVELOPMENT, INVITRO FERTILIZATION, BOVINE EMBRYOS, MEDIA, OVA, IMPLANTATION, BLASTOCYSTS, PROTEIN, MORULAE, STAGE
- Ankara Üniversitesi Adresli: Evet
Özet
In this study 33 New Zealand White does and 2 bucks were used. After induction Of Ovulation by human chorionic gonadotrophin (hCG) injection, oocytes were collected by flushing of oviduct. Oocytes and spermatozoa were incubated for 5 hours in Modifie Defined Medium (mDM) for fertilization and cultured in Ham's F-10 medium supplemented with 20 % Fetal Calf Serum (FCS) and 20 % Rabbit Serum (RS) for 18-21 hours, to evaluate the effect of serum in fertilization. Fertilization was assessed by detection of oocytes cleavage rates. The fertilization rates were recorded as 70.4 %, 73.8 %, 60.6 % in oocytes incubated in culture mediums with FCS or RS or without any serum respectively (p>0.05). Data were evaluated via Chi-square test. These data suggest that the differences between group averages were not statistically significant, although the fertilization rate in Ham's F-10 with rabbit serum was higher.