Akademik Veri Yönetim Sistemi
Bulletin of the Veterinary Institute in Pulawy, cilt.51, sa.2, ss.303-308, 2007 (SCI-Expanded)
The effects of artificial UVC radiation on some blood and urine parameters related to hepatic and renal functions in mice radiated with 30-watt UVC lamp (254 nm and 0.00014 J/cm2/s) were examined. Sixty female mice (Swiss Albino) were used as test animals. The animals were fed a mouse diet and water ad libitum and then exposed to artificial (JVC for 8 h daily. Blood and urine samples were taken before radiation (control) and at the 7th, 15 th, 30th, 45th, 60th and 75 th d after radiation exposure. The samples were collected in different pools. Urea, creatinine, cholesterol, ALT, AST, ALP, and GGT levels were determined in serum, and protein electrophoresis was made (SDS-PAGE) in urine samples. Significant increase (P<0.001) in serum urea values were observed on the 15th and 30th d of the exposure. Urea levels returned to the initial values. Increase in creatinine levels after 7th d of exposure and decrease until the 60th d (significant on the 15th and 45th d) were found. Creatinine levels increased again on the 75th d (P<0.001). Serum cholesterol and AST values were significantly increased (P<0.01) during the whole period of the exposure, except the 45th d. Serum ALT levels were not significantly affected during 30th d exposure. They decreased on the 45th d (P<0.01) and then increased on the 75 th d (P<0.01). Serum GGT and ALP levels were decreased during the whole period of the exposure (P<0.01), and GGT activity was at immeasurable levels on the 30th, 45th, and 60th d. Proteinuria, determined by microscopic observation of urine sediment, and the increase in serum urea and creatinine, observed on the 7th and 75th d of the exposure, were considered a renal disorder, and according to the electrophoretical pattern of urine proteins (SDS-PAGE) were defined as mostly glomcrulo-tubular type and glomerular type. This indicates that artificial UVC radiation significantly affected the renal function (P<0.01), while the hepatic functions were affected significantly (P<0.01) just on the 75th d of the exposure. Concerning the data, which coincide one on the top of the other, there was the opinion that the kidneys and liver showed different sensitivity to artificial UVC radiation.