Purification and characterization of intracellular and extracellular a-glucosidases from Geobacillus toebii strain E134


Cihan A., BENLİ M., Cokmus C.

CELL BIOCHEMISTRY AND FUNCTION, cilt.30, sa.1, ss.69-81, 2012 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 30 Sayı: 1
  • Basım Tarihi: 2012
  • Doi Numarası: 10.1002/cbf.1820
  • Dergi Adı: CELL BIOCHEMISTRY AND FUNCTION
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.69-81
  • Anahtar Kelimeler: thermostable, a-glucosidases, G, toebii E134, 16S rRNA gene, DNA-DNA hybridization, THERMOSTABLE ALPHA-GLUCOSIDASE, DEOXYRIBONUCLEIC-ACID, DNA HYBRIDIZATION, LIPID-COMPOSITION, RAPID METHOD, SUBSP NOV., BACTERIUM, EXO-ALPHA-1,4-GLUCOSIDASE, GLUCOHYDROLASES, ASSIGNMENT
  • Ankara Üniversitesi Adresli: Evet

Özet

Two different a-glucosidase-producing thermophilic E134 strains were isolated from a hot spring in Kozakli, Turkey. Based on the phenotypic, phylogenetic and chemotaxonomic evidence, the strain was proposed to be a species of G. toebii. Its thermostable exo-a-1,4-glucosidases also were characterized and compared, which were purified from the intracellular and extracellular fractions with estimated molecular weights of 65 and 45 kDa. The intracellular and extracellular a-glucosidases showed optimal activity at 65 degrees C, pH 7.0, and at 70 degrees C, pH 6.8, with 3.65 and 0.83 Km values for the pNPG substrate, respectively. Both enzymes remained active over temperature and pH ranges of 3570 degrees C and 4.511.0. They retained 82 and 84% of their activities when incubated at 60 degrees C for 5 h. Their relative activities were 4575% and 4560% at pH 4.5 and 11.0 values for 15 h at 35 degrees C. They could hydrolyse the a-1,3 and a-1,4 bonds on substrates in addition to a high transglycosylation activity, although the intracellular enzyme had more affinity to the substrates both in hydrolysis and transglycosylation reactions. Furthermore, although sodium dodecyl sulfate behaved as an activator for both of them at 60 degrees C, urea and ethanol only increased the activity of the extracellular a-glucosidase. By this study, G. toebii E134 strain was introduced, which might have a potential in biotechnological processes when the conformational stability of its enzymes to heat, pH and denaturants were considered. Copyright (C) 2011 John Wiley & Sons, Ltd.