Akademik Veri Yönetim Sistemi
KAFKAS UNIVERSITESI VETERINER FAKULTESI DERGISI, cilt.20, sa.4, ss.507-511, 2014 (SCI-Expanded, Scopus, TRDizin)
As a causal relationship between DNA damage, repair mechanism disorders and cancer demonstrated by experimental and epidemiological data. Transforming process starts from DNA damage to oxidative stress is associated with mutational inactivation of suppressor genes, activation of oncogenes. Related to this aim, it is important to indicate how cancer cells react under oxidative stress through by SOD2 expression, besides presenting correlation between NF-kB mechanism. Cell lines were maintained and cultured as recommended by ATCC (American type culture collection) resource. Cells were grouped and after detecting optimum concentration by MTT test (cell viability assay), treated with DNA damage agent (MNNG) and antioxidant (tempol) for indicated time points. Western blot analysis revealed that cell lines with comparable levels of SOD2 (Superokside dismutase 2) protein expression. However, cells were collected to measure NF-kB (Nuclear Factor kappa B) enzymatic activity using luciferase expression by transfection way. We observed high constitutive NF-kB activity by using 20 mu M MNNG although decreasing NF-kB during 30 mu M tempol treatment. The results showed strong correlation between SOD2 expression and NF-kB activation.