Cytotoxic Effects of Zinc Oxide on Human Periodontal Ligament Fibroblasts In Vitro

ŞEKER Ş.

Hittite Journal of Science and Engineering, vol.5, no.1, pp.1-5, 2018 (Peer-Reviewed Journal) identifier

  • Publication Type: Article / Article
  • Volume: 5 Issue: 1
  • Publication Date: 2018
  • Journal Name: Hittite Journal of Science and Engineering
  • Journal Indexes: TR DİZİN (ULAKBİM)
  • Page Numbers: pp.1-5
  • Ankara University Affiliated: Yes

Abstract

Zinc oxide (ZnO) has been widely used in wound dressing, food additive and root canalfilling material owing to its antibacterial and antifungal properties. This study describespotential cytotoxic effect of ZnO on human periodontal ligament fibroblasts (hPDLFs) in vitro.ZnO was characterized using BET (Braun-Emmet-Teller) and DLS (dynamic light scattering)analyses. The hPDLFs were treated with 0.1, 1, 10, 50 and 100 μg/mL of ZnO for 6 h, 24 hand 48 h durations. After exposure to ZnO, phase contrast microscopy, transmission electronmicroscopy (TEM), mitochondrial function (MTT) and lactate dehydrogenase (LDH)assays were used to evaluate cell morphology, particle uptake, cell viability and membraneleakage. In addition, real time cell responses to ZnO exposure were monitored by impedancemeasurements. Results indicated that ZnO exposure caused toxic effect in a dose- and timedependentmanner when compared to control group, especially at 50 and 100 μg/mL.