Cytotoxic Effects of Zinc Oxide on Human Periodontal Ligament Fibroblasts In Vitro

ŞEKER Ş.

Hittite Journal of Science and Engineering, cilt.5, sa.1, ss.1-5, 2018 (Hakemli Dergi) identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 5 Sayı: 1
  • Basım Tarihi: 2018
  • Dergi Adı: Hittite Journal of Science and Engineering
  • Derginin Tarandığı İndeksler: TR DİZİN (ULAKBİM)
  • Sayfa Sayıları: ss.1-5
  • Ankara Üniversitesi Adresli: Evet

Özet

Zinc oxide (ZnO) has been widely used in wound dressing, food additive and root canalfilling material owing to its antibacterial and antifungal properties. This study describespotential cytotoxic effect of ZnO on human periodontal ligament fibroblasts (hPDLFs) in vitro.ZnO was characterized using BET (Braun-Emmet-Teller) and DLS (dynamic light scattering)analyses. The hPDLFs were treated with 0.1, 1, 10, 50 and 100 μg/mL of ZnO for 6 h, 24 hand 48 h durations. After exposure to ZnO, phase contrast microscopy, transmission electronmicroscopy (TEM), mitochondrial function (MTT) and lactate dehydrogenase (LDH)assays were used to evaluate cell morphology, particle uptake, cell viability and membraneleakage. In addition, real time cell responses to ZnO exposure were monitored by impedancemeasurements. Results indicated that ZnO exposure caused toxic effect in a dose- and timedependentmanner when compared to control group, especially at 50 and 100 μg/mL.