Differential gene expression profiling of human adipose stem cells differentiating into smooth muscle-like cells by TGF beta 1/BMP4

Elçin A. E., Parmaksız M., Dogan A., Seker Ş., Durkut S., Dalva K., ...Daha Fazla

EXPERIMENTAL CELL RESEARCH, cilt.352, sa.2, ss.207-217, 2017 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 352 Sayı: 2
  • Basım Tarihi: 2017
  • Doi Numarası: 10.1016/j.yexcr.2017.02.006
  • Dergi Adı: EXPERIMENTAL CELL RESEARCH
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.207-217
  • Anahtar Kelimeler: Adipose stem cells, Differentiation, Smooth muscle cells, Microarray analysis, Differential transcriptome analysis, Vasculogenesis, THORACIC AORTIC-ANEURYSMS, APOLIPOPROTEIN-D, PROTEIN 4, INTERNATIONAL-SOCIETY, ARTICULAR-CARTILAGE, STROMAL CELLS, TISSUE, MATRIX, TRANSCRIPTOME, FIBROBLASTS
  • Ankara Üniversitesi Adresli: Evet

Özet

Regenerative repair of the vascular system is challenging from the perspectives of translational medicine and tissue engineering. There are fundamental hurdles in front of creating bioartificial arteries, which involve recaputilation of the three-layered structure under laboratory settings. Obtaining and maintaining smooth muscle characteristics is an important limitation, as the transdifferentiated cells fail to display mature phenotype. This study aims to shed light on the smooth muscle differentiation of human adipose stem cells (hASCs). To this end, we first acquired hASCs from lipoaspirate samples. Upon characterization, the cells were induced to differentiate into smooth muscle (SM)-like cells using a variety of inducer combinations. Among all, TGF beta 1/BMP4 combination had the highest differentiation efficiency, based on immunohistochemical analyses. hSM-like cell samples were compared to hASCs and to the positive control, human coronary artery-smooth muscle cells (hCA-SMCs) through gene transcription profiling. Microarray findings revealed the activation of gene groups that function in smooth muscle differentiation, signaling pathways, extracellular modeling and cell proliferation. Our results underline the effectiveness of the growth factors and suggest some potential variables for detecting the SM-like cell characteristics. Evidence in transcriptome level was used to evaluate the TGF beta 1/ BMP4 combination as a previously unexplored effector for the smooth muscle differentiation of adipose stem cells.