Restriction site mutation analysis in tobacco smoke exposed rats Sigara dumanina maruz birakilmis sicanlarda sinirli bolge mutasyon analizi

SÜZEN H. S., Parry J.

Acta Pharmaceutica Turcica, cilt.41, sa.4, ss.165-171, 1999 (Scopus) identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 41 Sayı: 4
  • Basım Tarihi: 1999
  • Dergi Adı: Acta Pharmaceutica Turcica
  • Derginin Tarandığı İndeksler: Scopus, International Pharmaceutical Abstracts
  • Sayfa Sayıları: ss.165-171
  • Anahtar Kelimeler: Polymerase chain reaction, Restriction site mutation assay technique, Tobacco smoke
  • Ankara Üniversitesi Adresli: Evet

Özet

Restriction site mutation (RSM) assay technique has been applied as a genotypic mutation analysis method in tobacco smoke exposed rats. In principle, the RSM assay technique may be used to analyze mutation induction in any organ, of any species, for which DNA sequence information is available. The RSM assay technique is a combination of two methods that of restriction enzyme digestion of DNA and amplification of resistant enzyme sequences using polymerase chain reaction (PCR). In this study, a number of parameters, which affect the two methods, were studied on p53 and ras genes. Several suitable restriction enzymes and primer pairs were identified for detecting mutations. The assay was carried out on 13 selected restriction enzyme recognition sequences located in the rat p53 and ras genes. No resistant amplification products have been observed in the selected restriction enzyme recognition sites in this study.