Determination of clenbuterol HCI in human serum, pharmaceuticals, and in drug dissolution studies by RP-HPLC

Ozkan, Y; Ozkan, SİBEL; Aboul-Enein, HY

doi:10.1081/jlc-100103403

Determination of clenbuterol HCI in human serum, pharmaceuticals, and in drug dissolution studies by RP-HPLC

Atıf İçin Kopyala

Ozkan Y., Ozkan S. A., Aboul-Enein H.

Journal of Liquid Chromatography and Related Technologies, cilt.24, sa.5, ss.679-691, 2001 (SCI-Expanded)

Yayın Türü: Makale / Tam Makale
Cilt numarası: 24 Sayı: 5
Basım Tarihi: 2001
Doi Numarası: 10.1081/jlc-100103403
Dergi Adı: Journal of Liquid Chromatography and Related Technologies
Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
Sayfa Sayıları: ss.679-691
Ankara Üniversitesi Adresli: Evet

Özet

A simple, reliable, and rapid RP-HPLC method has been developed for the determination of Clenbuterol HC1 in human serum and pharmaceuticals, in order to carry out drug dissolution studies for clenbuterol tablets. This compound is well separated on a C18 column by using the mobile phase consisting of a mixture of acetonitrile and ion-pair buffer (32:68; v/v) at a flow rate of 1.5 mL/min. Detection was carried out using a UV detector at 244 nm. ephedrine HC1 was used as an internal standard. Minimum detection limit obtained was 3.78 ng/mL. This method was applied, without any interferences from the excipients, for the determination of the drug in tablet formulation, human serum, and drug dissolution studies.