Effect of L-carnitine on the synthesis of nitric oxide in RAW 264•7 murine macrophage cell line

Koc A., ÖZKAN T., Karabay A. Z., SUNGUROĞLU A., Aktan F.

CELL BIOCHEMISTRY AND FUNCTION, sa.8, ss.679-685, 2011 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Basım Tarihi: 2011
  • Doi Numarası: 10.1002/cbf.1807
  • Dergi Adı: CELL BIOCHEMISTRY AND FUNCTION
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.679-685
  • Anahtar Kelimeler: L-carnitine, RAW 264 center dot 7, lipopolysaccharide (LPS), inducible nitric oxide synthase (iNOS), nuclear factor ?B (NF-?B), SELECTIVE INHIBITOR, SYNTHASE, INDUCTION, CANCER, ROLES
  • Ankara Üniversitesi Adresli: Evet

Özet

L-Carnitine (beta-hydroxy-gamma-trimethyl aminobutyric acid) plays a critical role in inflammatory diseases by modulating inflammatory cell functions. Inducible nitric oxide synthase (iNOS), a proinflammatory enzyme responsible for the generation of nitric oxide (NO), has been implicated in the pathogenesis of inflammatory diseases. Mechanism of action of L-carnitine on inflammation via iNOS and nuclear factor ?B (NF-?B) is unclear. In this study, we aimed to investigate the effect of L-carnitine on nitric oxide synthesis in lipopolysaccharide (LPS)-stimulated RAW 264 center dot 7 macrophage cells. For this purpose, cells were pretreated with various concentrations of L-carnitine and subsequently incubated with LPS (1 mu g center dot ml-1). NO levels, iNOS protein expression, and NF-?B activity were determined using colorimetric detection, Western blotting and transfection assays. Our results showed that treatment with L-carnitine suppressed nitric oxide production, iNOS protein expression and NF-alpha B activity. We demonstrated that inhibitory effect of L-carnitine on iNOS protein expression is at transcriptional level. This study may contribute to understanding the anti-inflammatory effect of L-carnitine. Copyright (c) 2011 John Wiley & Sons, Ltd.