Cryopreservation of rainbow trout (Oncorhynchus mykiss) semen

Tekin N., Secer S., AKÇAY E., Bozkurt Y.

ISRAELI JOURNAL OF AQUACULTURE-BAMIDGEH, cilt.55, sa.3, ss.208-212, 2003 (SCI-Expanded, Scopus) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 55 Sayı: 3
  • Basım Tarihi: 2003
  • Dergi Adı: ISRAELI JOURNAL OF AQUACULTURE-BAMIDGEH
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.208-212
  • Anahtar Kelimeler: cryopreservation, extender, fertilization, Oncorhynchus mykiss, semen, spermatological properties, SALMO-GAIRDNERI SEMEN, SPERMATOZOA, SPERM, FERTILIZATION, MOTILITY, STRAWS, CRYOPROTECTANT, FERTILITY, VOLUME
  • Ankara Üniversitesi Adresli: Evet

Özet

Rainbow trout semen was diluted in one of three extenders (1:3 v/v) containing egg yolk (7-10%) and 10% DMSO as cryoprotectants. The diluted semen was packaged in 0.5 ml straws and frozen in liquid nitrogen (LN2) vapor. For thawing, the straws were immersed in a water bath for 30 sec at 30degreesC. The best post-thaw motility and fertilization results, 56.67+/-5.77% and 80.5+/-16.01% respectively, were obtained when a glucose based extender was used.