Human sperm DNA damage in the context of assisted reproductive techniques

Youssry M., Ozme B., Orief Y., Zohni K., Al-Hasani S.

IRANIAN JOURNAL OF REPRODUCTIVE MEDICINE, vol.5, no.4, pp.137-150, 2007 (SCI-Expanded) identifier

  • Publication Type: Article / Review
  • Volume: 5 Issue: 4
  • Publication Date: 2007
  • Journal Name: IRANIAN JOURNAL OF REPRODUCTIVE MEDICINE
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Emerging Sources Citation Index (ESCI), Scopus, Academic Search Premier, CAB Abstracts, EMBASE, Veterinary Science Database, Directory of Open Access Journals
  • Page Numbers: pp.137-150
  • Keywords: fertilization, sperm DNA damage, assisted reproductive technique (ART), CHROMATIN-STRUCTURE ASSAY, DEOXYRIBONUCLEIC-ACID FRAGMENTATION, EJACULATED HUMAN SPERMATOZOA, IN-VITRO FERTILIZATION, OXIDATIVE STRESS, MALE-INFERTILITY, EMBRYO DEVELOPMENT, STRAND BREAKS, COMET ASSAY, HUMAN SEMEN
  • Ankara University Affiliated: No

Abstract

Fertilization involves direct interaction of the sperm and oocyte, fusion of the cell membranes and union of male and female gamete genomes. The completion of this process and subsequent embryo development depend in part on the inherent integrity of the sperm DNA. Sperm genome quality has been emphasized for several years as playing a major role in early embryogenesis. There is clinical evidence showing that human sperm DNA damage may adversely affect reproductive outcomes and that spermatozoa of infertile men possess substantially more DNA damage than do spermatozoa of fertile men. Testing DNA integrity may help selecting spermatozoa with intact DNA or with the least amount of DNA damage for use in assisted reproductive techniques (ARTs). This review will focus on how sperm DNA is organized, what causes sperm DNA damage and what impact this damage may have on reproductive outcome.