Akademik Veri Yönetim Sistemi
Biomass Conversion and Biorefinery, cilt.14, sa.9, ss.10611-10620, 2024 (SCI-Expanded)
Lignocellulosic biomass as a cheap and abundant raw material offers great advantages in terms of biotechnological applications. The current study aimed to produce bioethanol from mulberry pomace by newly isolated xylose/glucose co-fermenter Hanseniaspora uvarum. For this purpose, the xylose consumption capacity of the yeast was first tested using the synthetic xylose-containing medium as a carbon source, and Hanseniaspora uvarum effectively fermented xylose into ethanol. Afterward, Hansenispora uvarum was used for the fermentation of the liquid hydrolysate obtained by dilute acid pretreatment of mulberry pomace. Furthermore, a subsequent enzymatic hydrolysis of the pretreated mulberry pomace was performed to investigate its effect. For this purpose, initial biomass concentration (50–500 g/L) for fermentation was optimized in two different mulberry pomace media containing nitrogen sources or mineral salts. Enzymatic hydrolysis of pretreated mulberry pomace was performed at 400 g/L initial biomass loading with or without soluble soy protein addition, and a significant increase in fermentable sugar concentrations was observed. The highest sugar concentration was observed as 159.6 g/L when 400 g/L biomass loading was used during dilute acid pretreatment, and subsequent enzymatic hydrolysis was performed using 15 FPU/g cellulose. Moreover, after dilute acid pretreatment and enzymatic hydrolysis, ethanol production of Hanseniaspora uvarum reached 61.3 g/L (0.153 g/gbiomass) which is a sufficient amount for efficient distillation according to literature. Results show that xylose/glucose co-fermenter Hanseniaspora uvarum and mulberry pomace can be considered for second-generation bioethanol production.