Plasmid-mediated quinolone resistance in Salmonella serotypes isolated from chicken carcasses in Turkey

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Ata Z., YIBAR A., Arslan E., MÜŞTAK H. K., Gunaydin E.

ACTA VETERINARIA BRNO, cilt.83, sa.4, ss.281-286, 2014 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 83 Sayı: 4
  • Basım Tarihi: 2014
  • Doi Numarası: 10.2754/avb201483040281
  • Dergi Adı: ACTA VETERINARIA BRNO
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.281-286
  • Anahtar Kelimeler: Quinolone susceptibility, qnr, aac(6 ')-Ib-cr, real-time PCR, minimal inhibition concentration, ESCHERICHIA-COLI, ENTERICA, ANIMALS, FOOD, CIPROFLOXACIN, ENTERITIDIS, QNRS1
  • Ankara Üniversitesi Adresli: Evet

Özet

Quinolones have been extensively used for treatment of a variety of invasive and systemic infections of salmonellosis. Widespread use of these agents has been associated with the emergence and dissemination of quinolone-resistant pathogens. The quinolone resistance and plasmid-mediated quinolone resistance determinants (qnrA, qnrB, qnrS and aac(6')-Ib-cr) of 85 Salmonella isolates from chicken carcasses were investigated in this study. Isolates were serotyped according to the Kauffman-White-Le Minor scheme, and broth microdilution method was used to determine quinolone resistance. Plasmid-mediated quinolone resistance genes were investigated by real-time PCR and positive results were confirmed by sequencing. Among the Salmonella isolates, 30/85 (35%) and 18/85 (21%) were found to be resistant to enrofloxacin (MIC >= 2 mg/ml), and danofloxacin (MIC >= 2 mg/ml), respectively. All the isolates were negative for qnrA, qnrB and aac(6')-Ib-cr genes, nevertheless 2% (S. Brandenburg and S. Dabou) were positive for qnrS (qnrS1 determinant). This study is the first and unique investigating the plasmid-mediated quinolone resistance determinants of Salmonella isolated from chicken carcasses in Turkey.