Akademik Veri Yönetim Sistemi
Ankara Universitesi Eczacilik Fakultesi Dergisi, cilt.48, sa.1, ss.158-168, 2024 (Scopus)
Objective: Our study aimed to investigate the oxidative stress-mediated effect of wheat germ oil (WGO) on testicular damage caused by diabetes. Material and Method: 42 male Wistar albino rats were randomly divided into 6 groups: control, control low-dose (100 mg/kg/day), control high-dose (1000 mg/kg/day), diabetes control, diabetes low-dose (100 mg/kg/day), diabetic high-dose (1000 mg/kg/day). RY/carrier cornoil was applied to the diabetes and control groups by gavage for 28 days. Blood glucose levels were measured every week. Following euthanasia, testicular tissues were removed. Tissues were homogenized and total protein levels were measured by Bradford method. MDA levels were measured in tissues as an indicator of lipid peroxidation, and glutathione levels were measured as an indicator of oxidative stress. Result and Discussion: Both low and high dose WGO administration in the control group caused a decrease in relative testicular weight. The relative testicular weight of the high dose diabetic group decreased compared to the diabetic control group. There was no significant difference in blood glucose levels and body weights depending on WGO (p>0.05). Among diabetic animals, the lowest testicular MDA levels were found in the high -dose group. Glutathione levels increased in the control low-dose group. However, there was no significant difference between the groups in terms of MDA and glutathione levels (p>0.05). Further research is needed to investigate the effectiveness of WGO, which has a traditionally usage and antioxidant activity, on sexual hormones and other molecular pathways.