Effect of alternative cryopreservation procedures on bull semen

Creative Commons License

Yavas T. K., DAŞKIN A.

ANKARA UNIVERSITESI VETERINER FAKULTESI DERGISI, cilt.59, sa.3, ss.231-234, 2012 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 59 Sayı: 3
  • Basım Tarihi: 2012
  • Doi Numarası: 10.1501/vetfak_0000002531
  • Dergi Adı: ANKARA UNIVERSITESI VETERINER FAKULTESI DERGISI
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, TR DİZİN (ULAKBİM)
  • Sayfa Sayıları: ss.231-234
  • Anahtar Kelimeler: Bull, cryopreservation, semen, storage, ultra-low freezer, LIQUID-NITROGEN, MICROBIAL-CONTAMINATION, OF-152-DEGREES-C, ULTRAFREEZER, FREEZERS, EMBRYOS, BREED
  • Ankara Üniversitesi Adresli: Evet

Özet

The aim of this study was to assess the use of - 152 degrees C ultra freezer for freezing and storing bull semen as an alternative to freezing over the liquid nitrogen vapor and storing in liquid nitrogen in terms of certain post-thaw spermatological parameters. Two ejaculates, one per each bull, were used without pooling. Four different freezing and storing protocols were tested. At 1 week, 2 months and 6 months after cryopreservation, the frozen semen was thawed and the seminal parameters were determined. As a conclusion, this study has confirmed that the use of -152 degrees C ultra-low freezer for freezing and storing bull semen for 6 months is a viable alternative to liquid nitrogen.