Selective determination of non-organophosphorus insecticide using DNA aptamer-based single-use biosensors

Kocer M. B., AYDOĞDU TIĞ G., Pekyardimci S.

BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY, cilt.68, ss.1174-1184, 2021 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 68
  • Basım Tarihi: 2021
  • Doi Numarası: 10.1002/bab.2039
  • Dergi Adı: BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Applied Science & Technology Source, BIOSIS, Biotechnology Research Abstracts, CAB Abstracts, Chemical Abstracts Core, Compendex, Computer & Applied Sciences, EMBASE, Environment Index, Food Science & Technology Abstracts, INSPEC, MEDLINE, Veterinary Science Database
  • Sayfa Sayıları: ss.1174-1184
  • Anahtar Kelimeler: acetamiprid, aptamer, biosensor, electrochemistry, screen-printed electrode, GLASSY-CARBON ELECTRODE, LABEL-FREE, SENSITIVE DETECTION, ELECTROCHEMICAL APTASENSORS, ELECTROPOLYMERIZED FILM, LIQUID-CHROMATOGRAPHY, ACETAMIPRID RESIDUES, SENSOR, NANOMATERIALS, PERFORMANCE
  • Ankara Üniversitesi Adresli: Evet

Özet

In the present study, we developed a disposable aptamer-based biosensor for rapid, sensitive, and reliable detection of acetamiprid (ACE). To improve the sensitivity of the aptasensor, poly-5-amino-2-mercapto-1,3,4-thiadiazole [P(AMT)] and gold nanoparticles (AuNPs) were progressively electrodeposited on the screen-printed electrode (SPE) surface by using cyclic voltammetry (CV) technique. For the determination of ACE, thiol-modified primary aptamer (Apt1) was selected by using the SELEX method and immobilized on the surface of the P(AMT) and AuNPs-modified SPE (SPE/P(AMT)/AuNPs) via Au-S bonding. Then, the surface-bound aptamer was incubated with ACE for 45 Min. After that, the biotin-labeled aptamer 2 (Apt2) was interacted with the ACE, then the enzyme-labeled step was performed. In this step, alkaline phosphatase (ALP) was bound to the surface through the interaction between Apt2 labeled with biotin and streptavidin (strep)-ALP conjugate. The determination of ACE was achieved by measuring the oxidation signal of alpha-naphthol, which is formed on the electrode surface through the interaction of ALP with alpha-naphthyl phosphate. The working range of the developed aptasensor was determined as 5 x 10(-12)-5 x 10(-10 )mol L(-1)with a low limit of detection (1.5 pmol L-1). It was also found that the proposed aptasensor possessed great advantages such as low cost, good selectivity, and good reproducibility.