Evaluation of membrane integrity by hypo-osmotic swelling eosine test in canine spermatozoa

Uysal O., Korkmaz T.

INDIAN VETERINARY JOURNAL, vol.81, no.11, pp.1229-1231, 2004 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 81 Issue: 11
  • Publication Date: 2004
  • Journal Name: INDIAN VETERINARY JOURNAL
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.1229-1231
  • Ankara University Affiliated: Yes

Abstract

The integrity and functional activity of the sperm membrane is of major importance in the fertilization process and assessment of membrane function may be a useful indicator of the fertilizing ability of spermatozoa (Keel and Webster, 1990). To evaluate the characteristics of the spermatozoal membrane, Hypoosmotic Swelling-Eosine Test (HE-test) is the most suitable for the analysis of both the functional (fertilizing capabilities) and the structural integrity (viability) of the membrane in the same cells at the same time (Ramirez et al., 1992) and it is a more accurate semen analysis method in predicting the fertilizing capacity of a semen sample (Neild et al., 1998).