Electrooxidation of pimozide and its differential pulse voltammetric and HPLC-EC determination

Ozkan S. A., Ozkan Y., Senturk Z.

Analytica Chimica Acta, cilt.453, sa.2, ss.221-229, 2002 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 453 Sayı: 2
  • Basım Tarihi: 2002
  • Doi Numarası: 10.1016/s0003-2670(01)01412-x
  • Dergi Adı: Analytica Chimica Acta
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.221-229
  • Anahtar Kelimeler: pimozide, cyclic voltammetry, differential pulse voltammetry, high-performance liquid chromatography with electrochemical detection, carbon based electrodes, tablet, human serum, DRUGS
  • Ankara Üniversitesi Adresli: Evet

Özet

The oxidative behaviour of pimozide was studied in hydroalcoholic media (10 + 90 methanol-H2O, pH range 2-7.5) at carbon based electrodes. Pimozide was irreversibly oxidized at high positive potentials, resulting in the formation of a couple with a reduction and re-oxidation peak at much lower potentials. The response was evaluated with respect to pH, scan rate, addition of surfactant and other variables. Using differential pulse voltammetry (DPV), the drug yielded a well-defined voltammetric response in Britton-Robinson buffer, pH 2.1 at +1.1V (versus Ag/AgCl) on glassy carbon electrode. The process could be used to determine pimozide concentrations in the range 8 × 10-7-1 × 10-4M. Applicability to tablets and human serum analysis was illustrated. Furthermore, a high-performance liquid chromatographic method with electrochemical detection (HPLC-EC) was developed, which allowed pimozide to be detected down to a level of 2.7 × 10-10M (0.25ppb). © 2002 Elsevier Science B.V. All rights reserved.