Supercritical carbon dioxide extraction of Usnea longissima (L.) Ach.: Optimization by Box-Behnken design (BBD)

Creative Commons License

ATİLA DİNÇER C., Gokalp C., Getiren B., YILDIZ A., YILDIZ N.

TURKISH JOURNAL OF CHEMISTRY, vol.45, no.4, pp.1248-1256, 2021 (SCI-Expanded) identifier identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 45 Issue: 4
  • Publication Date: 2021
  • Doi Number: 10.3906/kim-2102-66
  • Journal Name: TURKISH JOURNAL OF CHEMISTRY
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Chemical Abstracts Core, TR DİZİN (ULAKBİM)
  • Page Numbers: pp.1248-1256
  • Keywords: Usnea longissima (L.) Ach., usnic acid (UA), supercritical extraction, response surface methodology (RSM), FLUID EXTRACTION, USNIC ACID, CO2 EXTRACTION, OPERATING-CONDITIONS, ANTIOXIDANT, LICHEN, SFE
  • Ankara University Affiliated: Yes

Abstract

Usnic acid (UA) was extracted from Usnea longissima (L.) Ach. in supercritical carbon dioxide (SC-CO2) medium. The selected process parameters were extraction temperature (35-45 degrees C), amount of co-solvent (0%-5%) and extraction time (5-9 h). These parameters were applied to Box-Behnken design (BBD) belonging to response surface methodology (RSM) to determine optimum process parameters for the highest amount of UA in the extract. g UA/100g lichen, extraction yield % and UA content values were calculated in the range of 0.045-0.317, 2.77-5.4 and 71%-82% in different experimental conditions, respectively. The optimum conditions were predicted as 42 degrees C, 4.3% (ethanol) and 7.48 h. It was determined that the predicted and experimental values of g UA/100g lichen were compatible, and the suggested model was valid.