GREEN SYNTHESIS AND CHARACTERIZATION OF SILVER AND ZINC NANOPARTICLES USING PYRACANTHA COCCINEA M.J. ROEM AND THEIR ANTICANCER ACTIVITIES

Gökdere N., Emir E., Bostancı H. E., Atakol O., Palabıyık İ. M.

18th International Nanoscience and Nanotechnology Conference, İstanbul, Türkiye, 26 - 28 Ağustos 2024, ss.155, (Özet Bildiri)

  • Yayın Türü: Bildiri / Özet Bildiri
  • Basıldığı Şehir: İstanbul
  • Basıldığı Ülke: Türkiye
  • Sayfa Sayıları: ss.155
  • Ankara Üniversitesi Adresli: Evet

Özet

GREEN SYNTHESIS AND CHARACTERIZATION OF SILVER AND ZINC NANOPARTICLES USING PYRACANTHA COCCINEA M.J. ROEM AND THEIR ANTICANCER ACTIVITIES

Nuran GÖKDERE 1,Erdal EMİR2,Hayrani Eren BOSTANCI3 ,Orhan ATAKOL2 ,İsmail Murat PALABIYIK 1

1Department of Analytical Chemistry, Faculty of Pharmacy, Ankara University, Ankara, Türkiye

Graduate School of Health Sciences, Ankara University, Ankara, TÜRKİYE

3 Department of Chemistry, Faculty of Science, Ankara University, Ankara, Türkiye

4Department of Biochemistry, Faculty of Pharmacy, Sivas Cumhuriyet University, Sivas, Türkiye

nutan.gokdere@gmail.com

 

Pyracantha coccinea M.J. Roem (Rosaceae) is a deciduous shrub native to Southeast Europe and Asia. The plant is rich in phenolics and flavonoids (Gül and Altuntaş, 2021) Nanoparticles synthesized by the green method are considered a clean, safe, inexpensive method according to traditional methods (physical and chemical methods) (Amini and Akbari, 2019). In this study, the phenolically rich Pyracantha coccinea M.J. Roem plant was extracted. The prepared statement was used to synthesize the zinc oxide nanoparticles (ZnONPs) and silver nanoparticles (AgNP) and investigated at the characterization and anticancer activity.

Methods

First, etanolic and methanolic extract from Pyracantha Coccinea M.Roem was prepared. Ethanolic extract was used for ZnONP synthesis, and methanolic extract was used for AgNP synthesis. The extract was mixed with the metal salt solution and the pH was adjusted to 10. It was incubated and centrifuged. Characterization studies were performed using UV-visible spectroscopy, fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD) and Scanning electron microscope (SEM). Anticancer activity was studied by MTT assay using human breast adenocarcinoma cell line (MCF7), rat glial tumor (C6), healthy mouse fibroblast cell line (L929) and colon cancer (HT29) cell lines (Bostancı et al., 2022).

Result

 The peak of AuNP, ZnONP and plant extract have been observed to occur at 417, 362 and 278nm, respectively. Functional groups on the NP surface were determined by FTIR and ZN-O peak at 669 cm-1 was observed. According to XRD, AgNP and ZnONP were found to have cubic (fcc) crystal structure and hexagonal structure, respectively. From the SEM, all NPs are less than 100nm. Nanoparticles did not show toxic effects on the C6 cell line, and NPs were found to be more effective in colon cancer than control.

Diccussion

Considering the characterization study, NPs were synthesized successfully. Considering the anticancer activity study, NPs were found to be effective against colon cancer.

References

·       Amini, S. M., & Akbari, A. 2019. Metal nanoparticles synthesis through natural phenolic acids. IET nanobiotechnology, 13(8), 771-777

·       Bostancı, H. E., Bilgiçli, A. T., Güzel, E., Günsel, A., Hepokur, C., Çimen, B., & Yarasir, M. N. (2022). Evaluation of the effects of newly synthesized metallophthalocyanines on breast cancer cell lines with photodynamic therapy. Dalton Transactions, 51(41), 15996-16008.

·       Gül, E. N., & Altuntaş, E. (2021). Ateş dikeni meyvesinin bazı biyo-teknolojik özelliklerinin belirlenmesi. Akademik Ziraat Dergisi, 10(2), 291-304.