Evaluation of auricular lymph node cell lymphocyte proliferation and cytokine production as non-radioactive endpoints during murine contact allergy

ÜLKER Ö., ATAK YÜCEL A., ATEŞ İ., Karakaya A.

JOURNAL OF IMMUNOTOXICOLOGY, vol.8, no.2, pp.131-139, 2011 (SCI-Expanded, Scopus)

  • Publication Type: Article / Article
  • Volume: 8 Issue: 2
  • Publication Date: 2011
  • Doi Number: 10.3109/1547691x.2010.547996
  • Journal Name: JOURNAL OF IMMUNOTOXICOLOGY
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.131-139
  • Keywords: Local lymph node assay, non-radioactive endpoints, DNCB, cytokine production, MOLECULAR-WEIGHT COMPOUNDS, INTERFERON-GAMMA, RESPIRATORY SENSITIZERS, CHEMICAL ALLERGENS, INTEGRATED MODEL, SKIN REACTIONS, ASSAY, MICE, HYPERSENSITIVITY, DIFFERENTIATION
  • Ankara University Affiliated: Yes

Abstract

The murine local lymph node assay (LLNA) has been developed as a test method to assess allergic contact dermatitis. In spite of the validity of the LLNA, attention was drawn to the two disadvantages: use of radioactivity for in vivo measurement of lymph node cell proliferation ([ 1, the applied concentrations of DNCB caused only allergic effect but not any irritant effect. This study reports that the use of these non-radioactive endpoints can assess allergic contact dermatitis caused by chemicals.