Akademik Veri Yönetim Sistemi
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS, 2023 (SCI-Expanded)
Biomolecular association of an anticancer drug, leflunomide (LEF) with human serum albumin (HSA), the leading ligands carrier in human circulation was characterized using biophysical (i.e., fluorescence, absorption and voltammetric) methods and computational (i.e., molecular docking and molecular dynamics simulation) techniques. Evaluations of fluorescence, absorption and voltammetric findings endorsed the complex formation between LEF and HSA. An inverse relationship of Stern-Volmer constant-temperature and hyperchromic shift of the protein's absorption signal with addition of LEF con-firmed the LEF quenched the HSA fluorescence through static process. Moderate nature of binding strength (binding constant = 2.76-4.77 x 10(4) M-1) was detected towards the LEF-HSA complexation, while the association process was naturally driven via hydrophobic interactions, van der Waals interactions and hydrogen bonds, as evident from changes in entropy (?S= + 19.91 J mol K--1(-1)) and enthalpy (?H = -20.09 kJ mol (-1)), and molecular docking assessments. Spectral analyses of synchron-ous and three-dimensional fluorescence validated microenvironmental fluctuations near Trp and Tyr residues upon LEF binding to the protein. LEF association with HSA significantly defended tempera-ture-induced destabilization of the protein. Although LEF was found to attach to HSA at Sudlow's sites I and II, but exhibited greater preference toward its site I, as detected by the investigations of com-petitive site-marker displacement. Molecular dynamics simulation assessment revealed that the com-plex attained equilibrium throughout simulations, showing the LEF-HSA complex constancy.