IN VITRO BULBLET INDUCTION FROM BULB SCALES OF ENDANGERED ORNAMENTAL PLANT MUSCARI AZUREUM

Uranbey S., Ipek A., Caliskan M., Dundar E., UZUN S., BAŞALMA D., ...Daha Fazla

BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT, cilt.24, sa.2, ss.1843-1848, 2010 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 24 Sayı: 2
  • Basım Tarihi: 2010
  • Doi Numarası: 10.2478/v10133-010-0024-4
  • Dergi Adı: BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.1843-1848
  • Anahtar Kelimeler: Muscari azureum, bulblet, micropropagation, bulb scale, REGENERATION, BENZYLADENINE, CULTURES
  • Ankara Üniversitesi Adresli: Evet

Özet

Muscari azureum with beautiful white and sky blue flowers is an important endangered ornamental plant of Turkey and needs exploitation for commercial propagation. 2-4 bulb scale explants of M. azureum were cultured in basal media supplemented with 2 mg/l 2,4-D, 20 g/l mannitol, 20 g/l sucrose, 0.5 mg/l NAA and different concentrations of BAP KIN, 2iP and TDZ plus 2 g/l gelrite. The best regeneration on 2 or 4 scales and the highest mean number of bulblets per explants (mean 8.77 per explant)" was achieved on an Orchimax medium supplemented with 2.0 mg/l BAP, 2 mg/l 2,4-D, 20 g/l mannitol, 20 g/l sucrose and 0.5 mg/l NAA for 2-scales. Mature bulblets were excised and individually rooted on half strength MS medium supplemented with I mg/l IBA, 0.5 g/l activated charcoal, 20 g/l sucrose and 6 g/l agar Regenerated plants from 2 and 4 scales were acclimatized with a 14% survival rate after 3 weeks.