Akademik Veri Yönetim Sistemi
Analytical and Quantitative Cytology and Histology, cilt.27, sa.3, ss.162-166, 2005 (SCI-Expanded)
OBJECTIVE: To describe the spectrofluorimetric method for the determination of ciprofloxacin hydrochloride in tablets under optimum conditions. STUDY DESIGN: Examination by spectrofluorimetry. RESULTS: The wavelengths of excitation and emission iuere 290 and 450 nm, respectively. The fluorescence intensity was linearly related to the drug concentration over the range 2-8 μg/mL, with a limit of detection of 1.4 μg/mL and a limit of quantitation of 2.0 μg/mL. The method was highly accurate and precise, having a relative SD of < 1.0%. The validity of the method was tested by recovery studies of the standard addition to pharmaceuticals, and the results were satisfactory. The results obtained by the application of this method and the official one were in good agreement, and statistical comparison by Student's t test and the variance ratio F test showed no significant difference between the 2 methods. CONCLUSION: The proposed method is simple and sufficiently precise for quality control purposes. © Science Printers and Publishers, Inc.