Association between delta-aminolevulinic acid dehydratase polymorphism and placental lead levels

Kayaalti Z., Sert S., AKYÜZLÜ D., Soylemez E., Soylemezoglu T.

ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY, cilt.41, ss.147-151, 2016 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 41
  • Basım Tarihi: 2016
  • Doi Numarası: 10.1016/j.etap.2015.11.017
  • Dergi Adı: ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.147-151
  • Anahtar Kelimeler: Delta-aminolevulinic acid dehydratase (ALAD), G177C single-nucleotide polymorphism, Lead, Placenta, Maternal blood, ALAD GENE POLYMORPHISMS, BLOOD LEAD, PORPHOBILINOGEN SYNTHASE, GENOTYPE, EXPOSURE, BIOMARKERS, SUSCEPTIBILITY
  • Ankara Üniversitesi Adresli: Evet

Özet

Lead inhibits the delta-aminolevulinic acid dehydratase (ALAD) activity and results in neurotoxic aminolevulinic acid accumulation in the blood. During pregnancy, lead in the maternal blood can easily cross the placenta. The aim of this study was to determine whether the maternal ALAD G177C polymorphism (rs1800435) was related to the placental lead levels. The study population comprised 97 blood samples taken from mothers to investigate ALAD G177C polymorphism and their placentas to measure lead levels. ALAD G177C polymorphism was detected by standard polymerase chain reaction (PCR)restriction fragment length polymorphism (RFLP) technique and atomic absorption spectrometry (AAS) equipped with a graphite furnace and Zeeman background correction system was used for lead determination. The median placental lead levels for ALAD1-1, ALAD1-2 and ALAD2-2 genotypes were 7.54 mu g/kg, 11.78 mu g/kg and 18.53 mu g/kg, respectively. Statistically significant association was found between the maternal ALAD G177C polymorphism and placental lead levels (p < 0.05). This study suggested that maternal ALAD G177C polymorphism was associated with placental lead levels. (C) 2015 Elsevier B.V. All rights reserved.