Akademik Veri Yönetim Sistemi
Microchemical Journal, cilt.226, 2026 (SCI-Expanded, Scopus)
Teriflunomide (TF) is a vital immunomodulatory agent used in the management of relapsing-remitting multiple sclerosis (RRMS). However, its standard quantification currently depends on high-cost chromatographic techniques and complex electrochemical sensors that involve intricate fabrication procedures, which often hinder routine clinical monitoring. There is a clear requirement for a more straightforward, sensitive, and economical analytical approach to facilitate widespread application. To address this need, this study presents the development and validation of an electrochemical sensing platform based on a multi-walled carbon nanotube-modified glassy carbon electrode (MWCNT/GCE). The modification of the GCE enhanced the electron transfer kinetics, as evidenced by the reduced peak-to-peak separation (ΔEp), which decreased from 0.246 V to 0.105 V, along with a significant drop in charge-transfer resistance (Rct) from 1450 Ω to 571 Ω. The electrochemical behavior of TF was investigated using differential pulse voltammetry (DPV) in Britton-Robinson buffer. Under optimized conditions (pH 8.0), TF exhibited a well-defined oxidation peak via diffusion-controlled mechanism. The proposed MWCNT/GCE demonstrated a linear response within a concentration range of 0.5 to 15.98 μM with a low limit of detection (LOD) of 0.033 μM. The sensor exhibited excellent selectivity in the presence of various interfering species, including dopamine, uric acid, and paracetamol (RSD < 1.05%). The practical applicability was successfully validated in pharmaceutical tablets, human plasma, and synthetic urine, yielding recoveries between 96.2% and 103.4%. Furthermore, statistical comparison with UV–Vis spectrophotometry using t-tests and F-tests at a 95% confidence level showed no significant differences, confirming the MWCNT/GCE as a reliable, cost-effective, and rapid alternative for routine quality control and clinical monitoring.