Akademik Veri Yönetim Sistemi
Journal of the Turkish Chemical Society, Section A: Chemistry, cilt.5, sa.3, ss.1295-1302, 2018 (Scopus)
© 2018, Turkish Chemical Society. All rights reserved.Poly(amidoamine) (PAMAM) dendrimers are good candidates for nucleic acid delivery with their well-defined characteristics. MicroRNA mediated regulation of biological process is also active an area of investigation. Fibroblast cells, such as MRC-5, are one of the cell lines used in biological researches due to their hard to transfect nature. In this two-staged study, cystamine core generation 5 PAMAM dendrimers were synthesized and fluorinated with pentafluoropropionic anhydride and subsequently tested as miRNA delivery reagent on MRC-5 cells. Effect of fluorination against to naked generation 5 dendrimer on transfection efficiency was also investigated by molecular docking and quantitative structure-activity relationship calculations. Structural characterization of the synthesized dendrimers was verified by spectroscopic techniques. Gel retardation assay, particle size and transmission electron microscopy results demonstrated polyplex formation of fluorinated dendrimers with miRNA at nanoscale level. Zeta potential values indicated non-aggregation and increased stability of the polyplexes. Prepared polyplexes with fluorinated dendrimer showed over 90% cell viability and transfection efficiency. In silico calculations confirmed the stable complexation with miRNA and good penetration capability into the cell.