Akademik Veri Yönetim Sistemi
4th International Congress "New Perspectives and Challenges of Sustainable Livestock Production, Belgrade, Sırbistan, 7 - 09 Ekim 2015, ss.470-480
Abstract: Despite progresses in in-vitro embryo production, the embryo block have still occurred in plenty of zygotes. The lack of understanding of developmental block mechanism and its relation with suboptimal IVEP conditions encouraged us to investigate the effect of somatic cell co-culturing with different culture media on this feature. The cows ovaries collected from local abattoir was transferred to laboratory to collect cumulus oocyte complexes (COCs). The selected COCs were matured in five media (TCM199, ham’s F12, mSOF, follicular fluid and chick embryo amniotic fluid) at 3 somatic cells monolayers (cumulus, granulose and oviduct epithelial cells) and without somatic cells in factorial design for 24 h under mineral oil. After IVF, the presumptive zygotes were cultured in own treatment which matured. The cleavage and block rate was recorded at days 2- 3 and 8 PI, respectively. The culturing medium containing ChEAF (chick embryonic amnion fluid) resulted in most cleavage rate (49.50±11.51) and least blocked zygotes (51.00±13.04) than other media (p<0.001) and cow’s oviductal epithelial cells (COECs) from the somatic cells factors was shown most cleavage rate (45.31±12.96) and least blocked zygotes (50.85±14.24) from other somatic cells (p<0.001). Zygotes which co-culture by COECs in ChEAF medium were significantly (p<0.001) different from other treatments in respect of cleavage rate (57.54±10.36) and decreasing of zygotic development block (41.31±5.98). In conclusion, the development of embryos are effected by in vitro culturing conditions and in this study ChEAF with cow oviductal epithelial cell resulted in low developmental block.
Key word: cleavage, co-culture, developmental block, media, zygote