Yuzuncu Yil University Journal of Agricultural Sciences, cilt.31, sa.2, ss.487-493, 2021 (Scopus)
© 2021, Centenary University. All rights reserved.Botrytis cinerea, a polyphagous pathogen, can infect all of the aboveground parts of tomato plants and cause significant yield and quality losses. Fungicides are commonly used for the control of this pathogen. Currently, resistance to fungicides, which provide the effective and fast control of pathogens, is an important problem. In this study, resistance of B. cinerea isolates obtained from tomato greenhouses in Antalya province against Signum® (boscalid + pyraclostrobin) and Cantus® (boscalid) fungicides were evaluated under in vitro conditions. Mycelium growth tests conducted with different fungicide concentrations and EC50 values were calculated. While EC50 values of isolates sensitive to boscalid varied between 0.7 and 8.6 μg/ml, EC50 values of isolates sensitive to boscalid + pyraclostrobin were found to be between 0.1 and 1.9 μg/ml. Conidial germination tests were carried out in a 2% water agar (WA) medium. It was determined that isolates 61, 69, 72, and 81 were resistant to both fungicides, while isolates 57 and 97 were sensitive to boscalid and resistant to boscalid + pyraclostrobin. It has been determined that 20% of the isolates were resistant to both fungicides. Isolates resistant to boscalid but sensitive to boscalid+pyraclostrobin were not found. With this current in vitro study, the first data on the resistance formation against boscalid and boscalid + pyraclostrobin active ingredients in B. cinerea populations in Antalya province were presented. There is a need to develop integrated control programs that can be used in the control of the pathogen.