Highly sensitive amperornetric biosensor for determination of NADH and ethanol based on Au-Ag nanoparticles/poly(L-Cysteine)/reduced graphene oxide nanocomposite

AYDOĞDU TIĞ G.

TALANTA, cilt.175, ss.382-389, 2017 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 175
  • Basım Tarihi: 2017
  • Doi Numarası: 10.1016/j.talanta.2017.07.073
  • Dergi Adı: TALANTA
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.382-389
  • Anahtar Kelimeler: NADH determination, Alcohol dehydrogenase, Au-Ag nanoparticles, Poly(L-cysteine), Reduced graphene oxide, NICOTINAMIDE ADENINE-DINUCLEOTIDE, GLASSY-CARBON ELECTRODE, ELECTROCATALYTIC OXIDATION, ALLOY NANOPARTICLES, REDUCTION, PLATFORM, FABRICATION, NANORODS, SENSOR
  • Ankara Üniversitesi Adresli: Hayır

Özet

This work presents the fabrication of a novel nicotinamide adenine dinucleotide (NADH) sensor using gold silver bimetallic nanoparticles (Au-AgNPs), poly(L-Cysteine) (P(L-Cys)) and electrochemically reduced graphene oxide (ERGO) modified glassy carbon electrode (GCE/Au-AgNPs/P(L-Cys)-ERGO). The composite electrode exhibited an excellent electrocatalytic response towards NADH at a low oxidation potential (+ 0.35 V) and minimization of surface contamination due to the synergistic effects of the Au-AgNPs, polymer and ERGO. Under optimum conditions, modified sensors allowed the detection of NADH with a wide linear range from 0.083 mu M to 1.05 mM with a low detection limit of 9.0 nM (S/N = 3). Moreover, this modified electrode was also used as a sensitive ethanol biosensor, which was prepared with alcohol dehydrogenase (ADH) via glutaraldehyde, bovin serum albumin and nafion (Naf). There was a linear response for ethanol in the concentration range from 0.017 to 1.845 mM with a low detection limit of 5.0 mu M (S/N = 3). The GCE/Au-AgNPs/P(L-Cys)-ERGO/ADH/Naf electrode can be successfully used for the determination of ethanol in different commercial beverages.