Construction and in vitro characterisation of aroA defective (aroA Delta) mutant Salmonella Infantis

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MÜŞTAK İ. B., YARDIMCI H.

ARCHIVES OF MICROBIOLOGY, cilt.201, sa.9, ss.1277-1284, 2019 (SCI-Expanded) identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 201 Sayı: 9
  • Basım Tarihi: 2019
  • Doi Numarası: 10.1007/s00203-019-01694-0
  • Dergi Adı: ARCHIVES OF MICROBIOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.1277-1284
  • Anahtar Kelimeler: aroA gene, Auxotrophic mutant, Recombinant vaccine, Salmonella Infantis, TYPHIMURIUM, LIVE, ENTERITIDIS, PERSISTENCE, STRAIN, MODEL
  • Ankara Üniversitesi Adresli: Evet

Özet

Poultry vaccine programs are important for control of Salmonella infections. Although there are vaccines for Salmonella Enteritidis, Salmonella Typhimurium and Salmonella Typhi, there are no vaccines for Salmonella Infantis which has an increased rate in the world. In this study, it was aimed to generate aroA gene deleted mutant bacteria for the constitution of S. Infantis vaccine prototype and the in vitro characterisation of this bacterium. S. Infantis auxotrophic mutant which has a block at any step of chorismate pathway has been constituted for the first time in the world and it was determined that this bacterium gets susceptibility against some antibiotics and antimicrobial substances. It was also observed that the adhesion and invasion rate of mutant strain tenfold decreased in comparison with the field strain in cell culture assay. It is understood from the in vitro evaluation of this mutant strain that it can be used as a vaccine candidate in further vaccine development studies.