Fast and versatile electrochemical approach for soft tissue decellularization

Ertuğrul M. İ., GÜRBÜZ A., ESKİZENGİN H., ODABAŞ S.

METHODSX, cilt.10, 2023 (ESCI) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 10
  • Basım Tarihi: 2023
  • Doi Numarası: 10.1016/j.mex.2023.102094
  • Dergi Adı: METHODSX
  • Derginin Tarandığı İndeksler: Emerging Sources Citation Index (ESCI), Scopus, BIOSIS, Directory of Open Access Journals
  • Anahtar Kelimeler: Biomaterials, Decellularization, Electro-chemical, Soft tissues, Tissue engineering
  • Ankara Üniversitesi Adresli: Evet

Özet

Decellularization is one of a promising technique in the field of biomaterials based on the idea of using an acellular construct, here the organ / tissue itself, as a biocompatible and biological construct. In the decellularization process, the main objective is to preserve the structural and functional properties while removing living cells. In the current paper, we describe an electro-chemical method for soft tissue decellularization at a specific voltages and time intervals, as well as further DNA, GAG, protein determinations, and histological examinations for the determination of decellularization efficacy. The approach proposed here, is: center dot Successful decellularization can be achieved by exposing the tissues to fewer chemicals than the traditional methods. center dot A facile and fast decellularization process long less than a day center dot An easy decellularization technique that may be applied to soft tissues