Novel voltammetric tumor necrosis factor-alpha (TNF-α) immunosensor based on gold nanoparticles involved in thiol-functionalized multi-walled carbon nanotubes and bimetallic Ni/Cu-MOFs

Yola M. L., Atar N.

ANALYTICAL AND BIOANALYTICAL CHEMISTRY, cilt.413, sa.9, ss.2481-2492, 2021 (SCI-Expanded, Scopus) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 413 Sayı: 9
  • Basım Tarihi: 2021
  • Doi Numarası: 10.1007/s00216-021-03203-z
  • Dergi Adı: ANALYTICAL AND BIOANALYTICAL CHEMISTRY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Aerospace Database, Aqualine, Aquatic Science & Fisheries Abstracts (ASFA), Artic & Antarctic Regions, BIOSIS, Biotechnology Research Abstracts, CAB Abstracts, Chemical Abstracts Core, Chimica, Communication Abstracts, Compendex, EMBASE, Food Science & Technology Abstracts, MEDLINE, Metadex, Pollution Abstracts, Veterinary Science Database, DIALNET, Civil Engineering Abstracts
  • Sayfa Sayıları: ss.2481-2492
  • Ankara Üniversitesi Adresli: Hayır

Özet

TNF-alpha, as a pro-inflammatory cytokine, regulates some physiological and pathological courses. TNF-alpha level increases in some important diseases such as cancer, arthritis, and diabetes. In addition, it displays an important function in Alzheimer's and cardiovascular diseases. Herein, a novel, sensitive, and selective voltammetric TNF-alpha immunosensor was prepared by using gold nanoparticles involved in thiol-functionalized multi-walled carbon nanotubes (AuNPs/S-MWCNTs) as sensor platform and bimetallic Ni/Cu-MOFs as sensor amplification. Firstly, the sensor platform was developed on glassy carbon electrode (GCE) surface by using mixture of thiol-functionalized MWCNTs (S-MWCNTs) and AuNPs. Then, capture TNF-alpha antibodies were conjugated to sensor platform by amino-gold affinity. After capture TNF-alpha antibodies' immobilization, a new-type voltammetric TNF-alpha immunosensor was developed by immune reaction between AuNPs/S-MWCNTs immobilized with primer TNF-alpha antibodies and bimetallic Ni/Cu-MOFs conjugated with seconder TNF-alpha antibodies. The prepared TNF-alpha immunosensor was characterized by transmission electron microscopy (TEM), scanning electron microscopy (SEM), x-ray diffraction (XRD) method, x-ray photoelectron spectroscopy (XPS), atomic force microscopy (AFM), thermogravimetric analysis, Fourier transform infrared spectroscopy (FTIR), cyclic voltammetry (CV), and electrochemical impedance spectroscopy (EIS). A linearity range of 0.01-1.0 pg mL(-1) and a low detection limit of 2.00 fg mL(-1) were also obtained for analytical applications.