Somatic embryogenesis from immature cotyledons of some European chestnut (Castanea sativa Mill.) cultivars

San B., Sezgin M., DUMANOĞLU H., Koksal A. I.

TURKISH JOURNAL OF AGRICULTURE AND FORESTRY, cilt.31, sa.3, ss.175-179, 2007 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 31 Sayı: 3
  • Basım Tarihi: 2007
  • Doi Numarası: 10.3906/tar-0611-9
  • Dergi Adı: TURKISH JOURNAL OF AGRICULTURE AND FORESTRY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, TR DİZİN (ULAKBİM)
  • Sayfa Sayıları: ss.175-179
  • Anahtar Kelimeler: chestnut, Castanea sativa Mill., somatic embryogenesis, AMERICAN CHESTNUT, EMBRYOS, GERMINATION, MATURATION, INVITRO, WALNUT
  • Ankara Üniversitesi Adresli: Evet

Özet

In 4 European chestnut (Castanea sativa Mill.) cultivars ('Haciibis ', 'Karamehmet', 'Osmanoglu' and 'Sariaslama'), somatic embryogenesis from immature cotyledons of open-pollinated seeds collected 5, 6, 7, and 8 weeks after anthesis and cultured on Driver and Kuniyuki walnut (DKW) medium was determined in this study. The percentage of embryogenic cotyledons and the number of embryos per embryogenic cotyledon were recorded at the end of the second subculture. The results showed that the highest percentage of embryogenesis ranged from 40% to 70% and the number of embryos per embryogenic cotyledon varied from 4.04 to 7.70 among the cultivars. Cotyledons collected 5, 6, and 7 weeks after anthesis were the most embryogenic.