Akademik Veri Yönetim Sistemi
JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES, cilt.40, sa.7, ss.333-339, 2017 (SCI-Expanded)
A new ultraperformance liquid chromatography (UPLC) method with photodiode array detection was developed for the quantitative analysis of a commercial syrup formulation containing ephedrine (EPH) and guaifenesin (GUA). In the development of UPLC method, experimental chromatographic conditions, flow rate, column temperature, and percentage of 0.1M H3PO4 in mobile phase, were optimized using chemometric multivariate strategy. From the application of a 3(3) full factorial design, the optimal chromatographic conditions were obtained as the flow rate of 0.29mL/min, column temperature of 36.4 degrees C, and 56.9% of 0.1M H3PO4 in the mobile phase. The optimal conditions gave us a good chromatographic separation of the analyzed drugs with short analysis runtime within 3min. Calibration curves for EPH and GUA in the linear working range of 4-64 and 6-96 mu g/mL, respectively, were obtained using peak areas detected at 215nm. Performance and validity of the optimized UPLC method were estimated by analyzing independent binary mixtures, inter-day and intra-day samples, and standard addition solutions containing EPH and GUA substances. It was concluded that the proposed method was a promising approach for the quantitative determination and routine analysis of a commercial syrup formulation of the titled substances.