The characterisation of the carbohydrate moieties of glycoproteins and the role of hydrazinolysis


SÜZEN S.

Fabad Journal of Pharmaceutical Sciences, vol.24, no.4, pp.233-239, 1999 (Scopus) identifier

  • Publication Type: Article / Review
  • Volume: 24 Issue: 4
  • Publication Date: 1999
  • Journal Name: Fabad Journal of Pharmaceutical Sciences
  • Journal Indexes: Scopus
  • Page Numbers: pp.233-239
  • Keywords: Carbohydrate, Glycoprotein, Hydrazinolysis
  • Ankara University Affiliated: Yes

Abstract

The characterisation of the carbohydrate moieties of glycoproteins requires the prior release of the oligosaccharides from the glycoprotein. The use of hydrazinolysis allows chemical release from glycoproteins of N-linked oligosaccharides but hydrazinolysis of glycopeptides containing serine (or threonine) bound oligosaccharides causes degradation of the oligosaccharides. Although it has been found that by using a lower temperature the degradation can be avoided, complete release of oligosaccharides from mucus glycoproteins which contain large numbers of oligosaccharides chains is still difficult to achieve.