Research Information System
Fabad Journal of Pharmaceutical Sciences, vol.24, no.4, pp.233-239, 1999 (Scopus)
The characterisation of the carbohydrate moieties of glycoproteins requires the prior release of the oligosaccharides from the glycoprotein. The use of hydrazinolysis allows chemical release from glycoproteins of N-linked oligosaccharides but hydrazinolysis of glycopeptides containing serine (or threonine) bound oligosaccharides causes degradation of the oligosaccharides. Although it has been found that by using a lower temperature the degradation can be avoided, complete release of oligosaccharides from mucus glycoproteins which contain large numbers of oligosaccharides chains is still difficult to achieve.