Differential diagnosis of Classical Swine Fever and Border Disease: Serological investigations of a pestivirus infection on a mixed farm

OĞUZOĞLU T. Ç., Floegel-Niesmann G., Frey H., Moennig V.

DEUTSCHE TIERARZTLICHE WOCHENSCHRIFT, cilt.108, sa.5, ss.210-213, 2001 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 108 Sayı: 5
  • Basım Tarihi: 2001
  • Dergi Adı: DEUTSCHE TIERARZTLICHE WOCHENSCHRIFT
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.210-213
  • Anahtar Kelimeler: differential diagnosis, pestivirus, Classical Swine Fever, Border Disease, serology, epidemiology, VIRAL DIARRHEA VIRUS, MONOCLONAL-ANTIBODIES, HOG-CHOLERA, PIGS, IDENTIFICATION
  • Ankara Üniversitesi Adresli: Evet

Özet

During recent years neutralising antibodies against Border Disease Virus (BDV) were found repeatedly in German pig herds. Consequently there was a demand for a differential diagnostic system. A permanent sheep cell line and BDV reference strain Moredun were chosen and were applyed in a could be used case study. A pestivirus could be isolated from piglets on a mixed farm and was characterised as,non-Classical Swine Fever' (CSF) by using monoclonal antibodies. Due to a CSF suspicion the pig herd was destroyed immediately. Serum samples of sheep from the same farm were used for further characterisation of the new virus isolate. A neutralisation test of the sheep sera was performed against different pestiviruses and the new isolate. Neutralising antibody titres against the new virus pig isolate were significantly higher than against all other pestiviruses. BDV strain Moredun recognised the antibodies clearly, whereas CSF viral strain Alfort 187 and several isolates of bovine viral diarrhoea virus (BVDV) strains scored the lowest cross reaction.