Akademik Veri Yönetim Sistemi
Turk Hijyen ve Deneysel Biyoloji Dergisi, cilt.72, sa.1, ss.45-58, 2015 (Scopus)
Objective: The taxonomy of Lecidea is extremely complex because of the enormous morphological variation within and between species. The aim of this study was to analyse the rDNA (ITS) regions of Lecidea species and related genus called Lecidella and Porpidia which are widely spreaded in Anatolia, Turkey. Methods: The ITS rDNA sequence information of 17 samples from 11 species which were collected from different provinces of Anatolia were generated. Some of the specimens from Lecidea, Lecidella, Lecanora and Porpidia genus were also taken from the GenBank (www.ncbi.nlm.nih.gov). The phylogenetic analysis was performed by the help of four different methods (NJ, ME, MP, UPGMA) and these different methods manifested similar results. Results: Minimum-Evolution (ME) dendrogram revealed that species of Lecidea, Lecidella, Porpidia and Ganoderma sp. genus were distributed into four main branches. Ganoderma applanatum (GU256764) which was considered as outgroup formed one of the branches, while the other species were collected on the other branchs. Generally the species which belong to the same genus, combined in one branch towards to the origin. In accordance with the results derived from molecular phylogenetic analysis, genus Lecidea is found closer Porpidia rather than Lecidella morphologically. Numerable 4100 nucleotides were obtained from DNA sequences of related region of studied samples. It was indicated that 177 nucleotides of those regions were stable (C), 747 nucleotides were variable (V). It was confirmed that there were transitions in 56 nucleotide pairs, tranversion in 54 nucleotide pairs of compared samples. Conclusion: In this study, the results of phylogenetic analysis of the genus Lecidea and other similar groups were firstly evaluated and the results will not be only a guide but also will provide a resource for next researchers.