Activities of DNA turn-over and free radical-metabolising enzymes and levels of peroxidation indices in human hepatic cancer tissues

Durak I., Karaayvaz M., ÖZTÜRK H. S., Kacmaz M., Akgul H.

CANCER RESEARCH THERAPY & CONTROL, cilt.5, sa.3, ss.195-201, 1998 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 5 Sayı: 3
  • Basım Tarihi: 1998
  • Dergi Adı: CANCER RESEARCH THERAPY & CONTROL
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.195-201
  • Anahtar Kelimeler: hepatic cancer, free radicals, DNA turn-over, peroxidation, ADENOSINE-DEAMINASE, SUPEROXIDE-DISMUTASE, XANTHINE-OXIDASE, CATALASE ACTIVITIES, OXYGEN RADICALS, HUMAN-EPIDERMIS, CARCINOMA, 5'-NUCLEOTIDASE, HEPATOMAS, TOXICITY
  • Ankara Üniversitesi Adresli: Evet

Özet

Activities of some DNA turn-over enzymes, namely adenosine deaminase (ADA), 5'nucleotidase (5'NT), guanase (GUA), xanthine oxidase (XO), xanthine dehidrogenase (XD), and hypoxanthine-guanine phosphoribosyl transferase (HGPRT) and those of free radical defense enzymes, namely superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and catalase (CAT) were measured in cancerous and non-cancerous human liver tissues. Levels of peroxidation indexes (PI), namely thiobarbituric acid-reagent substances (TBARS) and conjugated diens (CD) were also measured in the liver tissues. Of the DNA turn-over enzymes, ADA, XO and HGPRT activities in cancerous tissues were found higher, 5'NT unchanged, GUA and XD decreased compared with those of non-cancerous tissues. All of the free radical defense enzymes were found inhibited or repressed in the cancerous tissues. It has been found that the level of TEARS decreased but those of CD unchanged in the cancerous tissues. Correlation analysis results showed that enzymatic relations between enzyme activities were disordered in the cancerous tissues and mostly negative relations existed between the activities of the DNA turn-over and free radical defense enzymes.