Incidence of antibiotic resistance and virulence determinants in Enterococcus faecium and Enterococcus faecalis strains, isolated from traditional cheeses in Turkey

Yuksel F. N., AKÇELİK N., Akcelik M.

MOLECULAR GENETICS MICROBIOLOGY AND VIROLOGY, cilt.30, sa.4, ss.206-215, 2015 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 30 Sayı: 4
  • Basım Tarihi: 2015
  • Doi Numarası: 10.3103/s089141681504014x
  • Dergi Adı: MOLECULAR GENETICS MICROBIOLOGY AND VIROLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.206-215
  • Anahtar Kelimeler: Enterococcus faecalis, Enterococcus faecium, antibiotic resistance, plasmid DNA, virulence, ANTIMICROBIAL RESISTANCE, GLYCOPEPTIDE RESISTANCE, FECAL ENTEROCOCCI, FOOD, GENES, BACTERIOCINS, HUMANS, SYSTEM
  • Ankara Üniversitesi Adresli: Evet

Özet

Enterococcus faecalis (n = 15) and Enterococcus faecium (n = 33) strains isolated from traditional Turkish cheeses were tested for susceptibility to 11 different antimicrobial agents and for the presence of selected genes encoding resistance and 13 genes encoding virulence factors using PCR. Furthermore, the plasmid profile of enterococci was examined. All E. faecium and E. faecalis isolates were resistant to nalidixic acid and kanamycin. The percentages of other resistant E. faecium and E. faecalis isolates, respectively, were 97 and 100% to streptomycin, 15.1 and 20% to ampicillin, 9.1 and 26.7% to gentamycin, 12.1 and 46.6% to chloramphenicol, 12.1 and 60% to tetracycline, 75.7 and 93.3% to rifampicin, 84.8 and 80% to vancomycin, 97 and 100% to erytromycin and 72.7 and 60% to ciprofloxacin. efaA (fm) (100%) and ccf (90.1%) genes were the most common virulence genes identified among E. faecium isolates while efaA (fs) (100%), cpd (100%), ccf (93.3%) and cob (86.7%) genes among E. faecalis isolates. Cytolysin determinants (cylM, cylB, cylA) were not detected among tested strains. Plasmid profile analysis of Enterococcus spp. revealed plasmid DNA bands ranging in size from 2.4 to 35.8 kb.