Akademik Veri Yönetim Sistemi
MICxUS 2025 Symposium,, Lefkosia, Kıbrıs (Gkry), 21 - 23 Ocak 2025, ss.28-29, (Özet Bildiri)
Escherichia coli is one of the most common species involved in biofilm-associated diseases and it is
vital in urinary tract infections, causing relapses or chronic infections. The existence of multidrug-
resistant Escherichia coli bacteria is a global health threat imposing various disadvantages to
humans, livestock, food processing units, and the healthcare and pharmaceutical industries. Bacterial
biofilms are up to 1000 times more resistant to antibiotics than their planktonic counterparts and can
evade the effects of the host's immune system. The rising resistance to various antibiotics and the
high cost and lengthy process of developing new ones have intensified interest in natural compounds
as alternative therapeutic options. For these reasons, our planned study aimed to investigate the
antimicrobial and antibiofilm effects of natural products such as lactobionic acid and carvacrol on
pathogenic Escherichia coli species.
The broth microdilution method1 was used to investigate the Minimum Inhibitory Concentration
(MIC) of lactobionic acid and carvacrol against Escherichia coli ATCC 25922 wild-type strain and
fifty uropathogenic and catheter-associated isolates of Escherichia coli from different hospitals. Also,
the Minimum Bactericidal Concentration (MBC) of agents was determined. The effects of
lactobionic acid and carvacrol on eradicating mature E. coli biofilm structures were subsequently
assessed using crystal violet staining2
.
The Minimum Inhibitory Concentration (MIC) of carvacrol ranged from 256 to 4096 μg/mL, while
the MIC of lactobionic acid ranged from 2048 to 16,384 μg/mL. The findings revealed that carvacrol
and lactobionic acid posed bactericidal effects in 98% and 100% of Escherichia coli isolates,
respectively. The Minimum Biofilm Eradication Concentration (MBEC) of carvacrol and lactobionic
acid was determined, and experimental results revealed that carvacrol posed a moderate (24.05%)
antibiofilm effect on the samples. In contrast, lactobionic acid demonstrated a good antibiofilm effect
with an average biofilm reduction of 56.77% on E. coli isolates.
MICxUS 2025 Symposium, January 21-23, 2025
This research aims to raise awareness about the critical importance of biofilm prevention and
highlights the necessity to search for alternative natural therapies to overcome the growing challenge
of antibiotic resistance.