Flow Cytometric Aldehyde Dehydrogenase Assay Enables a Fast and Accurate Human Umbilical Cord Blood Hematopoietic Stem Cell Assessment

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Gencer E. B., Yurdakul P., DALVA K., BEKSAÇ M.

TURKISH JOURNAL OF HEMATOLOGY, cilt.34, sa.4, ss.314-320, 2017 (SCI-Expanded, Scopus, TRDizin) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 34 Sayı: 4
  • Basım Tarihi: 2017
  • Doi Numarası: 10.4274/tjh.2016.0214
  • Dergi Adı: TURKISH JOURNAL OF HEMATOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, TR DİZİN (ULAKBİM)
  • Sayfa Sayıları: ss.314-320
  • Anahtar Kelimeler: Cord blood, Aldehyde dehydrogenase, Colony-forming unit-granulocyte/macrophage, PROGENITOR CELLS, ENGRAFTMENT, TRANSPLANTATION, POPULATION, POTENCY, QUALITY
  • Açık Arşiv Koleksiyonu: AVESİS Açık Erişim Koleksiyonu
  • Ankara Üniversitesi Adresli: Evet

Özet

Objective: Colony-forming units of granulocytes/macrophages (CFU-GM) analysis is the most widely used method to determine the hematopoietic stem cell (HSC) content of human umbilical cord blood (CB) for prediction of engraftment potential. The measurement of aldehyde dehydrogenase (ALDH) activity is a more recent method for HSC qualification. Our aim was to correlate phenotypic and functional assays to find the most predictive method.