Development of a novel UPLC approach to co-prediction of four active compounds in a multi-component pharmaceutical preparation

ÜSTÜNDAĞ Ö., ÜÇER A., DİNÇ E.

Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, cilt.1240, 2024 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 1240
  • Basım Tarihi: 2024
  • Doi Numarası: 10.1016/j.jchromb.2024.124159
  • Dergi Adı: Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Aerospace Database, BIOSIS, CAB Abstracts, Chemical Abstracts Core, Chimica, Communication Abstracts, Compendex, Food Science & Technology Abstracts, MEDLINE, Metadex, Veterinary Science Database, Civil Engineering Abstracts
  • Anahtar Kelimeler: Chromatographic separation, Quaternary mixture, Simultaneous determination, UPLC-PDA
  • Ankara Üniversitesi Adresli: Evet

Özet

In this work, a new ultra-performance liquid chromatography method based on photodiode array detection (UPLC-PDA) was first developed for the quantitative analysis of the quaternary mixture of ascorbic acid (AA), paracetamol (PAR), caffeine (CAF) and chlorpheniramine maleate (CPA) in a commercial dosage form. The developed UPLC-PDA method offered a new possibility for the co-determination of four active ingredients in a drug combination with short run time and simple sample preparation. The successful chromatographic separation of the four drugs was performed using a Waters Acquity UPLC BEH C18 column (1.7 µm 2.1 × 100 mm) (Mildford, USA) and a mobile phase consisting of water (12 %), acetonitrile (13 %) and 0.1 M H3PO4 (75 %) at a flow rate of 0.25 mL/min. The validation of the proposed UPLC-PDA approach was verified by analyzing synthetic mixtures, inter- and intra-day experiments, and commercial powder samples and provided satisfactory results.