Detection of Mycoplasma ovipneumoniae and Pasteurella haemolytica antigens by an immunoperoxidase technique in pneumonic ovine lungs

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Haziroglu R., Diker K., Turkarslan J., Gulbahar M.

VETERINARY PATHOLOGY, vol.33, no.1, pp.74-76, 1996 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 33 Issue: 1
  • Publication Date: 1996
  • Doi Number: 10.1177/030098589603300108
  • Journal Name: VETERINARY PATHOLOGY
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.74-76
  • Keywords: immunoperoxidase, lambs, Mycoplasma ovipneumoniae, Pasteurella haemolytica, pneumonia, INFECTION, CALVES, BOVIS, VIRUS
  • Ankara University Affiliated: No

Abstract

Four hundred twenty pneumonic lungs from lambs were examined for Mycoplasma ovipneumoniae and Pasteurella haemolytica by an immunoperoxidase technique using an extravidin-biotin-peroxidase complex method in formalin-fixed, paraffin-embedded sections. Histologic examination of tissue sections revealed strong positive reactions in 60.9% and 68.3% of the lungs against M. ovipneumoniae and P, haemolytica, respectively. M. ovipneumoniae and P. haemolytica antigens were observed at the surface and/or within the epithelial cells, macrophages, leucocytes, and bronchiolar exudate. The location of M, ovipneumoniae in the cytoplasm of the epithelial cells and P, haemolytica in the neutrophils was detected immunohistochemically.