Osteogenic activities of MC3T3-E1 cells on heparin-immobilized poly(caprolactone) membranes

Gumusdereliolu M., KARAKEÇİLİ A., DEMİRTAŞ T. T.

Journal of Bioactive and Compatible Polymers, cilt.26, sa.3, ss.257-269, 2011 (SCI-Expanded) identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 26 Sayı: 3
  • Basım Tarihi: 2011
  • Doi Numarası: 10.1177/0883911511406329
  • Dergi Adı: Journal of Bioactive and Compatible Polymers
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.257-269
  • Anahtar Kelimeler: bone tissue engineering, covalent immobilization, heparin, osteogenic activity, poly(caprolactone)
  • Ankara Üniversitesi Adresli: Evet

Özet

The aim of this study was to investigate the effects of heparin on the activity of osteoblast-like cells seeded on poly(caprolactone) (PCL) membranes. The membranes were prepared by solvent-casting technique in ∼150 μm thickness. Then they were treated with 1,6-hexanediamine solution and functionalized with covalently bound heparin. The morphology, proliferation, and differentiation of MC3T3-E1 preosteoblasts on these membranes were investigated in vitro. The heparin functionalized PCL membranes, compared to non-functionalized membranes, significantly stimulated osteoblast proliferation. The Scanning electron microscope images confirmed the stimulative effect of covalently bound heparin on the osteoblast-like cell proliferation. The alkaline phosphatase and osteocalcin levels for cells proliferated on heparin containing PCL membranes were higher than that of nonfunctionalized membranes. © 2011 The Author(s).